Year: 2022 | Month: April | Volume 12 | Issue 2

Detection of Virulence Genes in Methicillin Resistant Staphylococcus aureus from Bovine Subclinical Mastitic Milk

Manisha Doot Abhishek Gaurav Sudeep Solanki Dinesh M Chavhan Vipin Chand Bairwa
DOI:10.30954/2277-940X.02.2022.5

Abstract:

Subclinical mastitis is a crucial problem influencing dairy animals across the globe. Staphylococcus aureus is generally considered to be a contagious udder pathogen, which mainly spreads within and between cows at milking. It can also be transmitted through the consumption of raw milk and milk products. Methicillin-resistant Staphylococcus aureus (MRSA) is a major concern for human health which can cause severe infectious diseases in humans. In the present study, bovine subclinical mastitic milk samples were processed for the isolation, identification and molecular characterization of the methicillin resistant Staphylococcus aureus. In total, 400 quarter milk samples were collected from 10 organized dairy farms of Udaipur district, Rajasthan from June 2021 to October 2021. These samples were screened for subclinical mastitis through Modified California Mastitis Test (MCMT) and Somatic Cell Count (SCC). Out of the 85 S. aureus isolates obtained from bovine subclinical mastitic milk (n=133), 5 isolates were confirmed as MRSA on the basis of phenotypic antibiotic susceptibility pattern giving a prevalence rate of 5.89%. All the MRSA isolates (n=5) were found to be positive for species specific (23S rRNA) and thermonuclease gene (nuc). Prevalence of virulence genes viz., clfA, fnbA and hlb in MRSA isolates was 60%, 80% and 40%, respectively. The presence of these virulent MRSA strains in milk indicates a potential public health risk.

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